Catalog Number - 68112-10P-2

Cogent RP C12™ is a unique reversed-phase UHPLC stationary phase featuring a rigid adamantane-like bonded phase on ultra-high purity silica. Fully double end capped and compatible with 100% aqueous mobile phases, it delivers excellent retention stability, rapid re-equilibration, and highly reproducible performance. With hydrophobic retention typically greater than C8 phases and lower than C18 phases, RP C12™ is an excellent choice for high-throughput screening, gradient methods, pharmaceutical analysis, and complex mixture separations.

68112-10P-2 is a UHPLC column packed with 1.8 µm RP C12™ particles with a 100 Å pore size in a 2.1 mm ID x 100 mm format. This is one of the most popular RP C12™ column configurations due to its combination of high efficiency, reduced solvent consumption, and excellent compatibility with LC-MS instrumentation. The 100 mm length provides enhanced resolution and peak capacity compared to shorter columns, while the sub-2 µm particle technology delivers outstanding chromatographic performance. Typical operating flow rates are approximately 0.2–0.4 mL/min, requiring an instrument capable of precise and reproducible low-flow operation.

Designed specifically for UHPLC systems, this column is ideal for high-throughput screening, impurity profiling, pharmaceutical analysis, and LC-MS methods involving complex sample matrices. The unique RP C12™ chemistry supports rapid equilibration and reliable retention even with highly aqueous mobile phases.

Key Benefits

• RP C12™ stationary phase

• Fully double end capped

• Adamantane-like bonded phase chemistry

• Compatible with 100% aqueous mobile phases

• Rapid re-equilibration between runs

• High-efficiency 1.8 µm particles

• Excellent for complex mixtures

• Increased peak capacity

• Optimized for LC-MS applications

• Reduced solvent consumption

Typical Applications

• UHPLC analysis

• LC-MS methods

• High-throughput screening (HTS)

• Pharmaceutical analysis

• Impurity profiling

• Complex mixture separations

• Gradient methods

• Quality control testing

• Stability studies

• Research and development

Specifications

• Product Type: UHPLC Column

• Stationary Phase: RP C12™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Adamantane-Like Carbon Silane

• Endcapping: Fully Double End Capped

• Particle Size: 1.8 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Typical Flow Rate: 0.2–0.4 mL/min

• Brand: Cogent

Important Notes

• Designed specifically for UHPLC operation and high-pressure reversed-phase chromatography

• 1.8 µm particles generate substantially higher backpressure than conventional HPLC columns

• Maximum performance is achieved on UHPLC systems designed for sub-2 µm particle technology

• Compatible with 100% aqueous mobile phases without loss of retention

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.4 mL/min

• The 100 mm length provides greater resolution and peak capacity than 50 mm columns

• Samples should be filtered through a 0.2 µm syringe filter prior to injection

• May be used on compatible HPLC systems only if instrument pressure limits are not exceeded and low-flow operation is reliable

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